Reporter

Part:BBa_K121010

Designed by: Masashi Kajita   Group: iGEM08_Tokyo_Tech   (2008-10-16)

Ptet GFP on pSB6

Promoter (tetR replessed) + RBS + GFP + TT on pSB6 (Part:BBa_I739201).

Pressure response

We measured pressure response of BBa_K121010 in BBa_I739003 in E. Coli strain JM109.

figure. Pressure response of Ptet. Ptet activity increased about 3-fold under 30 MPa ⇒[http://2008.igem.org/Team:Tokyo_Tech/Protocol Protocol is here]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 724


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Parameters
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