Pseudoknot from Infectious Bronchitis Virus that induces -1 frameshift during translation. Part has flanking RFC 25 prefix and suffix upstream and downstream.
RFC 25 prefix 5' GAATTCGCGGCCGCTTCTAGATGGCCGGC 3'
RFC 25 suffix 5' ACCGGTTAATACTAGTAGCGGCCGCTGCAG 3'
Assembly with this part should be done using the RFC 25 assembly strategy (which creates the following scar: 5' [part A] ACCGGC [part B] 3'). Assembling using RFC 23 (Silver standard) creates a stop codon in the -1 frame of the scar sequence (5' [part A] ACTAGA [part B] 3'), thus stopping translation in the -1 frame.
This part was characterized using a fluorescent protein test construct (BBa_K1210000), and has been shown to have a frameshifting frequency of 21 ± 5%. This reported frameshifting frequency is in agreement with previously published values (Tholstrup et al., 2011)
Tholstrup, J., Oddershede, L. B., & Sørensen, M. A. (2011). mRNA pseudoknot structures can act as ribosomal roadblocks. Nucleic acids research, 40(1), 303-313.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC