Reporter construct for measuring the frameshifting efficiency of the PK401 pseudoknot. Produces a reporter of framshift-induction. If translation continues in the 0 frame, a stop codon will be reached to give CFP as the final product. If frameshifting occurs due to the PK401 pseudoknot, translation will continue in the -1 frame to produce a CFP-YFP fusion protein with which FRET can be measured.
"TAATAA" stop codons were removed from the end of eCFP
"TT" two nucleotide insertion was added after the pseudoknot to put eYFP into the -1 frame.
(1) Hansen T.M., Reihani S.N., Oddershede L.B., Sørensen M.A. (2007)Correlation between mechanical strength of messenger RNA pseudoknots and ribosomal frameshifting. Proc Natl Acad Sci 104(14), 5830-5.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC