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Part:BBa_K1207000

Designed by: Jeremiah Miller   Group: iGEM13_OU-Norman_OK   (2013-09-12)


Gram positive origin of replication

This part is an origin of replication for Gram positive organisms. This sequence has been used in plasmids for electrotransformation of Clostridia with success. It is necessary to have a functional origin of replication for the chassis organism; therefore, if the chassis organism used is a Gram positive, this sequence will be a necessary addition to the iGEM standard plasmid backbones in order to allow for the replication of the plasmid.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None