Coding

Part:BBa_K1205002

Designed by: Blake Wilhelmsen   Group: iGEM13_UIUC_Illinois   (2013-09-16)

CBCWV Carnitine/Betaine/Choline Uptake Protein

This part encodes for an uptake protein in the cellular membrane which takes in carnitine, betaine, and choline.

We analyzed its function through the use of liquid chromatography-mass spectrometry.

LCMS Data for CbcWV and CaiX.

As you can see from our data, the amount of L-carnitine suspended in the media of our cells was decreased drastically as our cells grew. When comparing controls of cells with the backbone only, we found no change in the L-carnitine concentration of the media.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None