Regulatory

Part:BBa_K1204023

Designed by: Matthew Baron   Group: iGEM13_Duke   (2013-09-16)


TEF1pr-BS3x-20B

Same as BBa_K1204017 but binding site is repeated three times to create apparent cooperativity in the binding of the corresponding iTALs and gRNA/dCas9 complex.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 583
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 583
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 583
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 583
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 184


[edit]
Categories
Parameters
n/aTEF1pr-BS3x-20B