Regulatory

Part:BBa_K1204022

Designed by: Matthew Baron   Group: iGEM13_Duke   (2013-09-16)


TEF1pr-BS3x-20A

Same as BBa_K1204016 except the binding site is repeated three time to create apparent cooperativity with the binding of the iTALs and gRNA/dCas9 complex

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 174


[edit]
Categories
Parameters
n/aTEF1pr-BS3x-20A