Reporter

Part:BBa_K1204018

Designed by: Matthew Baron   Group: iGEM13_Duke   (2013-09-16)


TEF1pr-BS1x-20C

Same as BBa_K1204013 but binding site is 20 base pairs instead of 16. Binding site designed to bind iTAL and gRNA/dCas9 complex 20C.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 575
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 575
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 575
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 575
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 176


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Categories
Parameters
None