Reporter

Part:BBa_K1204013

Designed by: Matthew Baron   Group: iGEM13_Duke   (2013-09-16)


TEF1pr-BS1x-16A

This is a strong constitutive promoter in yeast with a binding site downstream. The binding site is designed to be directly targeted by our iTAL 16A or gRNA 16A. When the binding site is occupied by an iTAL or gRNA/dCas9 complex, the expression of the YFP reporter should be knocked down.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 521
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 176


[edit]
Categories
Parameters
None