Reporter
Part:BBa_K1204013
Designed by: Matthew Baron Group: iGEM13_Duke (2013-09-16)
TEF1pr-BS1x-16A
This is a strong constitutive promoter in yeast with a binding site downstream. The binding site is designed to be directly targeted by our iTAL 16A or gRNA 16A. When the binding site is occupied by an iTAL or gRNA/dCas9 complex, the expression of the YFP reporter should be knocked down.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 521
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 176
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Categories
Parameters
None |