DNA

Part:BBa_K1195011

Designed by: Lindsey Perry, Xiuqi Li   Group: iGEM13_BYU_Provo   (2013-10-25)


T7 Bacteriophage Minor Capsid Protein (Small Mutant 2)

This is the gene that encodes the minor capsid protein in wild type T7 bacteriophage. The major capsid protein can also be found in this gene, but it is shorter. (The minor capsid protein results from a -1 translation shift towards the end of the major capsid protein.) These two capsid proteins make up the external part of T7 bacteriophage's capsid. We mutated this gene so that it produces a capsid smaller than normal.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 292
    Illegal BsaI.rc site found at 496


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