Composite

Part:BBa_K1194003

Designed by: Rohan Bendre, Aman Kumar, Namit Holay, Mitan Sutradhar   Group: iGEM13_IIT_Madras   (2013-09-23)


pLuxR --> I3A gene

The I3A gene is actually a polycistronic construct composed of two genes coding for two enzymes - tryptophan transaminase and indole pyruvate decarboxylase. The two DNA constructs are joined by a linker sequence. This entire assembly is placed downstream of an N-acyl homoserine lactone (AHL) inducible promoter pLuxR.

The I3A genes function to convert tryptophan to indole-3-acetaldehyde (I3A). Tryptophan transaminase converts tryptophan to indole pyruvate while indole pyruvate decarboxylase converts indole pyruvate to I3A. I3A is a known biofilm inhibitor.

In the future, other teams can use either of our two enzymes as there is a restriction enzyme site incorporated in the linker sequence. The part will be useful in any project involving biosynthesis of indole and its derivatives.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 11
    Illegal PstI site found at 3027
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 11
    Illegal PstI site found at 3027
    Illegal NotI site found at 1213
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 269
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 11
    Illegal PstI site found at 3027
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 11
    Illegal PstI site found at 3027
    Illegal AgeI site found at 327
    Illegal AgeI site found at 690
    Illegal AgeI site found at 1787
    Illegal AgeI site found at 2301
    Illegal AgeI site found at 2571
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None