Part:BBa_K1194001
pLuxR --> I3A gene
The I3A gene is actually a polycistronic construct composed of two genes coding for two enzymes - tryptophan transaminase and indole pyruvate decarboxylase. The two DNA constructs are joined by a linker sequence. This entire assembly is placed downstream of an N-acyl homoserine lactone (AHL) inducible promoter pLuxR. The I3A genes function to convert tryptophan to indole-3-acetaldehyde (I3A). Tryptophan transaminase converts tryptophan to indole pyruvate while indole pyruvate decarboxylase converts indole pyruvate to I3A. I3A is a known biofilm inhibitor. In the future, other teams can use either of our two enzymes as there is a restriction enzyme site incorporated in the linker sequence. The part will be useful in any project involving biosynthesis of indole and its derivatives.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 253
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 311
Illegal AgeI site found at 674
Illegal AgeI site found at 1767
Illegal AgeI site found at 2281
Illegal AgeI site found at 2551 - 1000COMPATIBLE WITH RFC[1000]
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