Coding

Part:BBa_K1189034

Designed by: Taylor Remondini   Group: iGEM13_Calgary   (2013-10-24)

J04500_GFP_freiburg This is a the standard GFP reporter (E0040) that contains the prefix/suffix required for RFC25 standard assembly (K648013) placed behind an IPTG inducible promoter (J04500). This part allows for the easy construction and expression of fusion proteins containing GFP based on the Freiburg fusion standard (Muller et al,, 2007).


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 870


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