Tag

Part:BBa_K1188003

Designed by: William Heymann   Group: iGEM13_CU-Boulder   (2013-09-05)


FMN-binding protein

This part is a short (~370 bp) flavin mononucleotide (FMN)-binding protein that has Freiburg restriction sites in order to create fusion proteins. This can be used to tag proteins for the purpose of characterizing the amount of expressed protein, which can be measured by the absorption of visible light at 375 and 440 nm.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal AgeI site found at 373
  • 1000
    COMPATIBLE WITH RFC[1000]


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