Coding

Part:BBa_K1187001

Designed by: James Ellinger   Group: iGEM13_Minnesota   (2013-09-17)


Human insulin gene, optimized for expression in Pichia pastoris

The sequence we used for insulin was obtained from NCBI. It is the coding sequence for human insulin. The sequence obtained from NCBI was codon optimized for use in P. pastoris utilizing IDT codon optimization software. Expressing and obtaining pure has insulin from Pichia cultures will reduce allergic responses, inconsistent purity and concentration, and host rejection in patients who take insulin as a treatment of type I and type II diabetes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None