Primer

Part:BBa_K1182401

Designed by: Muhammad Hanifi   Group: iGEM13_UI-Indonesia   (2013-09-16)

[Forward] Split β-galactosidase - ω fragment

Forward primer for amplifying omega fragment only of beta-galactosidase enzyme from full-length LacZ (BBa_K823016) in BioBrick format (with prefix and suffix)

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 5
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5
    Illegal NotI site found at 11
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 5
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 5
    Illegal XbaI site found at 20
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/a[Forward] Split β-galactosidase - ω fragment