Primer
Part:BBa_K1182401
Designed by: Muhammad Hanifi Group: iGEM13_UI-Indonesia (2013-09-16)
[Forward] Split β-galactosidase - ω fragment
Forward primer for amplifying omega fragment only of beta-galactosidase enzyme from full-length LacZ (BBa_K823016) in BioBrick format (with prefix and suffix)
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 5
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 5
Illegal NotI site found at 11 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 5
- 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 5
- 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 5
Illegal XbaI site found at 20 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
n/a | [Forward] Split β-galactosidase - ω fragment |