Composite

Part:BBa_K118025

Designed by: Chris French   Group: iGEM08_Edinburgh   (2008-10-23)

limonene generator with lac promoter

This part consists of a lac promoter (with lacZ' tag for blue-white selection) coupled to the limonene generation system consisting of dxs (deoxyxylulose phosphate synthase, rate controlling step of terpenoid biosynthesis in bacteria) + LIMS1 (limonene synthase 1 from lemon) + appY (a transcription factor known to increase terpenoid biosynthesis). See the description of BBa_K118024 for more information about the components.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1354
    Illegal SapI.rc site found at 2614


[edit]
Categories
//function/biosynthesis/isoprenoid
Parameters
None