Signalling

Part:BBa_K1180003

Designed by: Chen Xi, Niu Yuchen,Dai Yimei   Group: iGEM13_NJU_China   (2013-09-24)


Fusion protein of lamp 2b and pre-s1 for liver targeting of the exosome

Lamp 2b (lysosomal associated membrane protein 2b) is a protein ubiquitously expressed on the surface of the exosomes. By genetically engineer a target protein to the outmembrane part of the lamp 2b, we can use the lamp 2b to bring our target protein to the surface of the exosome. Thus we can endow the exosome with site-specificity by the addition of the fusion protein.For liver targeting, we need to first find a protein specifically recognize hepatic cells. Since Heptitis B virus can infect hepatic cells distinctively, and from recent study, we know that HBV recognizes the hepatic cells via the interaction between the pre-S1 of the HBV envelop protein and NTCP receptor of the hepatic cells.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1390
    Illegal SpeI site found at 703
    Illegal PstI site found at 1087
    Illegal PstI site found at 1473
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 703
    Illegal PstI site found at 1087
    Illegal PstI site found at 1473
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1872
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1390
    Illegal SpeI site found at 703
    Illegal PstI site found at 1087
    Illegal PstI site found at 1473
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1390
    Illegal SpeI site found at 703
    Illegal PstI site found at 1087
    Illegal PstI site found at 1473
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 334
    Illegal BsaI site found at 1567


[edit]
Categories
Parameters
n/aFusion protein of lamp 2b and pre-s1 for liver targeting of the exosome