Generator
Part:BBa_K1179083
Designed by: Nelson Hall Group: iGEM13_MIT (2013-10-03)
CAG_Cas9-VN155
Description
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1730
Illegal EcoRI site found at 5644
Illegal EcoRI site found at 6471
Illegal XbaI site found at 1634
Illegal PstI site found at 2065
Illegal PstI site found at 4033
Illegal PstI site found at 4267
Illegal PstI site found at 5479
Illegal PstI site found at 5783
Illegal PstI site found at 6190 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1730
Illegal EcoRI site found at 5644
Illegal EcoRI site found at 6471
Illegal PstI site found at 2065
Illegal PstI site found at 4033
Illegal PstI site found at 4267
Illegal PstI site found at 5479
Illegal PstI site found at 5783
Illegal PstI site found at 6190 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1730
Illegal EcoRI site found at 5644
Illegal EcoRI site found at 6471
Illegal BglII site found at 2072
Illegal BglII site found at 3009
Illegal BamHI site found at 1778
Illegal BamHI site found at 3827
Illegal XhoI site found at 12
Illegal XhoI site found at 5365 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1730
Illegal EcoRI site found at 5644
Illegal EcoRI site found at 6471
Illegal XbaI site found at 1634
Illegal PstI site found at 2065
Illegal PstI site found at 4033
Illegal PstI site found at 4267
Illegal PstI site found at 5479
Illegal PstI site found at 5783
Illegal PstI site found at 6190 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1730
Illegal EcoRI site found at 5644
Illegal EcoRI site found at 6471
Illegal XbaI site found at 1634
Illegal PstI site found at 2065
Illegal PstI site found at 4033
Illegal PstI site found at 4267
Illegal PstI site found at 5479
Illegal PstI site found at 5783
Illegal PstI site found at 6190
Illegal NgoMIV site found at 1322
Illegal NgoMIV site found at 1479
Illegal NgoMIV site found at 4561 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |