Part:BBa_K1179075

TRE-t_eYFP-4x503

This part is submitted under the RFC 65 standard.

This encodes for the doxycycline regulated expression of yellow fluorescent protein and the sequence has four miRNA503 target sites appended to it.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 6
  Illegal EcoRI site found at 322
  Illegal EcoRI site found at 1233
  Illegal XbaI site found at 30
  Illegal SpeI site found at 1209
  Illegal PstI site found at 590
  Illegal PstI site found at 1117
  Illegal PstI site found at 1140
  Illegal PstI site found at 1163
  Illegal PstI site found at 1186
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 6
  Illegal EcoRI site found at 322
  Illegal EcoRI site found at 1233
  Illegal SpeI site found at 1209
  Illegal PstI site found at 590
  Illegal PstI site found at 1117
  Illegal PstI site found at 1140
  Illegal PstI site found at 1163
  Illegal PstI site found at 1186
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 6
  Illegal EcoRI site found at 322
  Illegal EcoRI site found at 1233
  Illegal BamHI site found at 353
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 6
  Illegal EcoRI site found at 322
  Illegal EcoRI site found at 1233
  Illegal XbaI site found at 30
  Illegal SpeI site found at 1209
  Illegal PstI site found at 590
  Illegal PstI site found at 1117
  Illegal PstI site found at 1140
  Illegal PstI site found at 1163
  Illegal PstI site found at 1186
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 6
  Illegal EcoRI site found at 322
  Illegal EcoRI site found at 1233
  Illegal XbaI site found at 30
  Illegal SpeI site found at 1209
  Illegal PstI site found at 590
  Illegal PstI site found at 1117
  Illegal PstI site found at 1140
  Illegal PstI site found at 1163
  Illegal PstI site found at 1186
  
• 1000
  COMPATIBLE WITH RFC[1000]