Part:BBa_K1179074

Hef1a_eYFP-4x503

This part is submitted under the RFC 65 standard.

This encodes for the constitutive expression of yellow fluorescent protein and the sequence has four miRNA503 target sites appended to it.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 2077
  Illegal SpeI site found at 2053
  Illegal PstI site found at 315
  Illegal PstI site found at 820
  Illegal PstI site found at 1434
  Illegal PstI site found at 1961
  Illegal PstI site found at 1984
  Illegal PstI site found at 2007
  Illegal PstI site found at 2030
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 2077
  Illegal SpeI site found at 2053
  Illegal PstI site found at 315
  Illegal PstI site found at 820
  Illegal PstI site found at 1434
  Illegal PstI site found at 1961
  Illegal PstI site found at 1984
  Illegal PstI site found at 2007
  Illegal PstI site found at 2030
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 2077
  Illegal BglII site found at 569
  Illegal BamHI site found at 1197
  Illegal XhoI site found at 968
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 2077
  Illegal SpeI site found at 2053
  Illegal PstI site found at 315
  Illegal PstI site found at 820
  Illegal PstI site found at 1434
  Illegal PstI site found at 1961
  Illegal PstI site found at 1984
  Illegal PstI site found at 2007
  Illegal PstI site found at 2030
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 2077
  Illegal SpeI site found at 2053
  Illegal PstI site found at 315
  Illegal PstI site found at 820
  Illegal PstI site found at 1434
  Illegal PstI site found at 1961
  Illegal PstI site found at 1984
  Illegal PstI site found at 2007
  Illegal PstI site found at 2030
  Illegal NgoMIV site found at 703
  Illegal AgeI site found at 81
  
• 1000
  COMPATIBLE WITH RFC[1000]