Generator

Part:BBa_K1179073

Designed by: Nelson Hall   Group: iGEM13_MIT   (2013-09-18)


TRE-tight_eYFP-4x451

This generates an eYFP transcript which can be repressed by miRNA 451. Under the regulation of the TRE promoter and when cotransfected with rtTA it can be induced by the presence of Doxycycline.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 1229
    Illegal XbaI site found at 30
    Illegal SpeI site found at 1205
    Illegal PstI site found at 590
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 1229
    Illegal SpeI site found at 1205
    Illegal PstI site found at 590
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 1229
    Illegal BamHI site found at 353
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 1229
    Illegal XbaI site found at 30
    Illegal SpeI site found at 1205
    Illegal PstI site found at 590
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 1229
    Illegal XbaI site found at 30
    Illegal SpeI site found at 1205
    Illegal PstI site found at 590
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None