Coding

Part:BBa_K1179064

Designed by: Kyle Lathem   Group: iGEM13_MIT   (2013-09-15)

LacI-4x503ts

This part is submitted under the RFC 65 standard.

It is the coding sequence for the DNA-binding protein LacI that inhibits the expression of genes being driven by a Lac promoter by binding to the Lac Operon. It also has four miRNA503 target sites for controlled translational repression.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1224
    Illegal PstI site found at 1132
    Illegal PstI site found at 1155
    Illegal PstI site found at 1178
    Illegal PstI site found at 1201
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1224
    Illegal PstI site found at 1132
    Illegal PstI site found at 1155
    Illegal PstI site found at 1178
    Illegal PstI site found at 1201
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1224
    Illegal PstI site found at 1132
    Illegal PstI site found at 1155
    Illegal PstI site found at 1178
    Illegal PstI site found at 1201
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1224
    Illegal PstI site found at 1132
    Illegal PstI site found at 1155
    Illegal PstI site found at 1178
    Illegal PstI site found at 1201
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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