Coding

Part:BBa_K1179061

Designed by: Kyle Lathem   Group: iGEM13_MIT   (2013-09-15)

eYFP-4x503ts

This part is submitted under the RFC 65 standard.

It is the coding sequence of yellow fluorescent protein appended with four miRNA503 target sites for controlled micro RNA repression.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 881
    Illegal SpeI site found at 857
    Illegal PstI site found at 238
    Illegal PstI site found at 765
    Illegal PstI site found at 788
    Illegal PstI site found at 811
    Illegal PstI site found at 834
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 881
    Illegal SpeI site found at 857
    Illegal PstI site found at 238
    Illegal PstI site found at 765
    Illegal PstI site found at 788
    Illegal PstI site found at 811
    Illegal PstI site found at 834
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 881
    Illegal BamHI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 881
    Illegal SpeI site found at 857
    Illegal PstI site found at 238
    Illegal PstI site found at 765
    Illegal PstI site found at 788
    Illegal PstI site found at 811
    Illegal PstI site found at 834
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 881
    Illegal SpeI site found at 857
    Illegal PstI site found at 238
    Illegal PstI site found at 765
    Illegal PstI site found at 788
    Illegal PstI site found at 811
    Illegal PstI site found at 834
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None