Coding

Part:BBa_K1179060

Designed by: Kyle Lathem   Group: iGEM13_MIT   (2013-09-15)

eYFP-4x451ts

This part is submitted under the RFC 65 standard.

It is the coding sequence of yellow fluorescent protein appended with four miRNA451 target sites for controlled micro RNA repression.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 877
    Illegal SpeI site found at 853
    Illegal PstI site found at 238
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 877
    Illegal SpeI site found at 853
    Illegal PstI site found at 238
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 877
    Illegal BamHI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 877
    Illegal SpeI site found at 853
    Illegal PstI site found at 238
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 877
    Illegal SpeI site found at 853
    Illegal PstI site found at 238
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None