Coding

Part:BBa_K1179057

Designed by: Brandon Nadres   Group: iGEM13_MIT   (2013-09-15)

VN173-Cas9(D10A and H840A mutant)

This part is submitted under the RFC 65 standard. It's a fusion of Cas9 and the N-terminal split venus fluorescent protein. The Venus fluorescence protein is an improved YFP construct that was created by modifying GFP and has been split at the 172th residue. The N-terminal venus can fuse with the C-terminal venus to produce yellow fluorescence.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4401
    Illegal EcoRI site found at 4762
    Illegal PstI site found at 243
    Illegal PstI site found at 822
    Illegal PstI site found at 2790
    Illegal PstI site found at 3024
    Illegal PstI site found at 4236
    Illegal PstI site found at 4540
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4401
    Illegal EcoRI site found at 4762
    Illegal PstI site found at 243
    Illegal PstI site found at 822
    Illegal PstI site found at 2790
    Illegal PstI site found at 3024
    Illegal PstI site found at 4236
    Illegal PstI site found at 4540
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4401
    Illegal EcoRI site found at 4762
    Illegal BglII site found at 829
    Illegal BglII site found at 1766
    Illegal BamHI site found at 1
    Illegal BamHI site found at 2584
    Illegal XhoI site found at 4122
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4401
    Illegal EcoRI site found at 4762
    Illegal PstI site found at 243
    Illegal PstI site found at 822
    Illegal PstI site found at 2790
    Illegal PstI site found at 3024
    Illegal PstI site found at 4236
    Illegal PstI site found at 4540
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4401
    Illegal EcoRI site found at 4762
    Illegal PstI site found at 243
    Illegal PstI site found at 822
    Illegal PstI site found at 2790
    Illegal PstI site found at 3024
    Illegal PstI site found at 4236
    Illegal PstI site found at 4540
    Illegal NgoMIV site found at 3318
    Illegal AgeI site found at 4734
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None