Coding

Part:BBa_K1179054

Designed by: Brandon Nadres   Group: iGEM13_MIT   (2013-09-15)

VN155-Cas9(D10A and H840A mutant)

This part is submitted under the RFC 65 standard. It's a fusion of Cas9 and the N-terminal split venus fluorescent protein. The Venus fluorescence protein is an improved YFP construct that was created by modifying GFP and has been split at the 154th residue. The N-terminal venus can fuse with the c-terminal venus to produce yellow fluorescence.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4347
    Illegal EcoRI site found at 4708
    Illegal PstI site found at 243
    Illegal PstI site found at 768
    Illegal PstI site found at 2736
    Illegal PstI site found at 2970
    Illegal PstI site found at 4182
    Illegal PstI site found at 4486
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4347
    Illegal EcoRI site found at 4708
    Illegal PstI site found at 243
    Illegal PstI site found at 768
    Illegal PstI site found at 2736
    Illegal PstI site found at 2970
    Illegal PstI site found at 4182
    Illegal PstI site found at 4486
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4347
    Illegal EcoRI site found at 4708
    Illegal BglII site found at 775
    Illegal BglII site found at 1712
    Illegal BamHI site found at 1
    Illegal BamHI site found at 2530
    Illegal XhoI site found at 4068
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4347
    Illegal EcoRI site found at 4708
    Illegal PstI site found at 243
    Illegal PstI site found at 768
    Illegal PstI site found at 2736
    Illegal PstI site found at 2970
    Illegal PstI site found at 4182
    Illegal PstI site found at 4486
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4347
    Illegal EcoRI site found at 4708
    Illegal PstI site found at 243
    Illegal PstI site found at 768
    Illegal PstI site found at 2736
    Illegal PstI site found at 2970
    Illegal PstI site found at 4182
    Illegal PstI site found at 4486
    Illegal NgoMIV site found at 3264
    Illegal AgeI site found at 4680
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None