Part:BBa_K1179051

VC155-Cas9(D10A and H840A mutant)

This part is submitted under the RFC 65 standard. It's a fusion of Cas9 and the C-terminal split venus fluorescent protein. The Venus fluorescence protein is an improved YFP construct that was created by modifying GFP and has been split at the 154th residue. The C-terminal venus can fuse with VN155 or VN173 to produce yellow fluorescence.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 4137
  Illegal EcoRI site found at 4498
  Illegal PstI site found at 558
  Illegal PstI site found at 2526
  Illegal PstI site found at 2760
  Illegal PstI site found at 3972
  Illegal PstI site found at 4276
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 4137
  Illegal EcoRI site found at 4498
  Illegal PstI site found at 558
  Illegal PstI site found at 2526
  Illegal PstI site found at 2760
  Illegal PstI site found at 3972
  Illegal PstI site found at 4276
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 4137
  Illegal EcoRI site found at 4498
  Illegal BglII site found at 565
  Illegal BglII site found at 1502
  Illegal BamHI site found at 1
  Illegal BamHI site found at 2320
  Illegal XhoI site found at 3858
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 4137
  Illegal EcoRI site found at 4498
  Illegal PstI site found at 558
  Illegal PstI site found at 2526
  Illegal PstI site found at 2760
  Illegal PstI site found at 3972
  Illegal PstI site found at 4276
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 4137
  Illegal EcoRI site found at 4498
  Illegal PstI site found at 558
  Illegal PstI site found at 2526
  Illegal PstI site found at 2760
  Illegal PstI site found at 3972
  Illegal PstI site found at 4276
  Illegal NgoMIV site found at 3054
  Illegal AgeI site found at 4470
  
• 1000
  COMPATIBLE WITH RFC[1000]