Coding

Part:BBa_K1179049

Designed by: Kyle Lathem   Group: iGEM13_MIT   (2013-09-15)

VC155

This part is submitted under the RFC 65 standard.

The Venus fluorescence protein is an improved YFP construct that was created by modifying GFP. The protein has been split at the 154th residue. The C-terminal venus can fuse with VN155 or VN173 to produce yellow fluorescence. We express the split venus constructs with a constitutive CAG promoter. A single split venus construct can not reconstitute to form a functional protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None