Generator

Part:BBa_K1179040

Designed by: Lauren St. Hilaire   Group: iGEM13_MIT   (2013-09-15)

TRE_Cas9-VP16

Inducible expression of Cas9-VP16. Used for doxycycline-controlled amounts of activation of promoters with VP16.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 3695
    Illegal XbaI site found at 30
    Illegal PstI site found at 2084
    Illegal PstI site found at 2318
    Illegal PstI site found at 3530
    Illegal PstI site found at 3834
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 3695
    Illegal PstI site found at 2084
    Illegal PstI site found at 2318
    Illegal PstI site found at 3530
    Illegal PstI site found at 3834
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 3695
    Illegal BglII site found at 1060
    Illegal BamHI site found at 1878
    Illegal XhoI site found at 3416
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 3695
    Illegal XbaI site found at 30
    Illegal PstI site found at 2084
    Illegal PstI site found at 2318
    Illegal PstI site found at 3530
    Illegal PstI site found at 3834
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 3695
    Illegal XbaI site found at 30
    Illegal PstI site found at 2084
    Illegal PstI site found at 2318
    Illegal PstI site found at 3530
    Illegal PstI site found at 3834
    Illegal NgoMIV site found at 2612
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None