Generator

Part:BBa_K1179033

Designed by: Lauren St. Hilaire   Group: iGEM13_MIT   (2013-09-15)

CMV_2xKMet-eGFP

Constitutive expression of green fluorescent protein flanked with 2 k-turn binding sites for L7Ae translational repression of the fluorescent protein.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 680
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 714
    Illegal BamHI site found at 744
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 750
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None