Generator

Part:BBa_K1179032

Designed by: Lauren St. Hilaire   Group: iGEM13_MIT   (2013-09-15)

CMV_ L7Ae_myc-His6

Constitutive expression of translational repressor protein L7Ae appended with a six histidine tag for use in protein purification.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1037
    Illegal XbaI site found at 1051
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1037
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1037
    Illegal XhoI site found at 1045
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1037
    Illegal XbaI site found at 1051
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1037
    Illegal XbaI site found at 1051
    Illegal AgeI site found at 1105
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None