Part:BBa_K1179030

Cas9-VN173 (D10A and H840A mutant)

Coding sequence of Cas9-VN173 fusion protein. The VN173 split venus fluorescent protein is being fused to the Cas9 in order to be able to target multiple sequences of DNA.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 3867
  Illegal EcoRI site found at 4748
  Illegal PstI site found at 288
  Illegal PstI site found at 2256
  Illegal PstI site found at 2490
  Illegal PstI site found at 3702
  Illegal PstI site found at 4006
  Illegal PstI site found at 4413
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 3867
  Illegal EcoRI site found at 4748
  Illegal PstI site found at 288
  Illegal PstI site found at 2256
  Illegal PstI site found at 2490
  Illegal PstI site found at 3702
  Illegal PstI site found at 4006
  Illegal PstI site found at 4413
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 3867
  Illegal EcoRI site found at 4748
  Illegal BglII site found at 295
  Illegal BglII site found at 1232
  Illegal BamHI site found at 1
  Illegal BamHI site found at 2050
  Illegal XhoI site found at 3588
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 3867
  Illegal EcoRI site found at 4748
  Illegal PstI site found at 288
  Illegal PstI site found at 2256
  Illegal PstI site found at 2490
  Illegal PstI site found at 3702
  Illegal PstI site found at 4006
  Illegal PstI site found at 4413
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 3867
  Illegal EcoRI site found at 4748
  Illegal PstI site found at 288
  Illegal PstI site found at 2256
  Illegal PstI site found at 2490
  Illegal PstI site found at 3702
  Illegal PstI site found at 4006
  Illegal PstI site found at 4413
  Illegal NgoMIV site found at 2784
  
• 1000
  COMPATIBLE WITH RFC[1000]