Generator

Part:BBa_K1179027

Designed by: Lauren St. Hilaire   Group: iGEM13_MIT   (2013-09-15)

CAG_tagBFP

Constitutive expression of bright blue fluorescent protein.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1730
    Illegal EcoRI site found at 2581
    Illegal XbaI site found at 1634
    Illegal XbaI site found at 2624
    Illegal PstI site found at 2586
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1730
    Illegal EcoRI site found at 2581
    Illegal PstI site found at 2586
    Illegal NotI site found at 2475
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1730
    Illegal EcoRI site found at 2581
    Illegal BamHI site found at 2612
    Illegal XhoI site found at 12
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1730
    Illegal EcoRI site found at 2581
    Illegal XbaI site found at 1634
    Illegal XbaI site found at 2624
    Illegal PstI site found at 2586
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1730
    Illegal EcoRI site found at 2581
    Illegal XbaI site found at 1634
    Illegal XbaI site found at 2624
    Illegal PstI site found at 2586
    Illegal NgoMIV site found at 1322
    Illegal NgoMIV site found at 1479
    Illegal AgeI site found at 1769
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2403


[edit]
Categories
Parameters
None