Generator

Part:BBa_K1179026

Designed by: Lauren St. Hilaire   Group: iGEM13_MIT   (2013-09-15)

CAG_mKate-PEST

Constitutive expression of red fluorescent protein appended with a PEST sequence for rapid degradation.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1730
    Illegal EcoRI site found at 2652
    Illegal XbaI site found at 1634
    Illegal XbaI site found at 2648
    Illegal PstI site found at 2602
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1730
    Illegal EcoRI site found at 2652
    Illegal PstI site found at 2602
    Illegal NotI site found at 2637
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1730
    Illegal EcoRI site found at 2652
    Illegal XhoI site found at 12
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1730
    Illegal EcoRI site found at 2652
    Illegal XbaI site found at 1634
    Illegal XbaI site found at 2648
    Illegal PstI site found at 2602
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1730
    Illegal EcoRI site found at 2652
    Illegal XbaI site found at 1634
    Illegal XbaI site found at 2648
    Illegal PstI site found at 2602
    Illegal NgoMIV site found at 1322
    Illegal NgoMIV site found at 1479
    Illegal NgoMIV site found at 2528
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2433
    Illegal SapI.rc site found at 1815


[edit]
Categories
Parameters
None