Generator

Part:BBa_K1179023

Designed by: Lauren St. Hilaire   Group: iGEM13_MIT   (2013-09-15)

CAG_Acyl-TyA-rtTA3-6xHis

This part was created for the RFC 65 standard. This part is the constitutive expression of Acyl-TyA-rtTA with an appended His tag. This is exported out of the cell and into exosomes, the his tag allowing for protein extraction from the exosomes.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1730
    Illegal XbaI site found at 1634
    Illegal PstI site found at 2379
    Illegal PstI site found at 2628
    Illegal PstI site found at 2650
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1730
    Illegal PstI site found at 2379
    Illegal PstI site found at 2628
    Illegal PstI site found at 2650
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1730
    Illegal BglII site found at 2479
    Illegal XhoI site found at 12
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1730
    Illegal XbaI site found at 1634
    Illegal PstI site found at 2379
    Illegal PstI site found at 2628
    Illegal PstI site found at 2650
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1730
    Illegal XbaI site found at 1634
    Illegal PstI site found at 2379
    Illegal PstI site found at 2628
    Illegal PstI site found at 2650
    Illegal NgoMIV site found at 1322
    Illegal NgoMIV site found at 1479
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2312
    Illegal BsaI.rc site found at 2664
    Illegal BsaI.rc site found at 3117


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Categories
Parameters
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