Generator

Part:BBa_K1179021

Designed by: Lauren St. Hilaire   Group: iGEM13_MIT   (2013-09-15)

CAG_Acyl-TyA-mKate-6xHis fusion protein

This part encodes the expression of red fluorescent protein appended with a His tag and also has a exportation sequence. This allows the fluorescent protein to be exported from the cells into exosomes and the his tag allows for protein extraction from the exosomes.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1730
    Illegal XbaI site found at 1634
    Illegal PstI site found at 2359
    Illegal PstI site found at 2608
    Illegal PstI site found at 2630
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1730
    Illegal PstI site found at 2359
    Illegal PstI site found at 2608
    Illegal PstI site found at 2630
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1730
    Illegal BglII site found at 2459
    Illegal XhoI site found at 12
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1730
    Illegal XbaI site found at 1634
    Illegal PstI site found at 2359
    Illegal PstI site found at 2608
    Illegal PstI site found at 2630
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1730
    Illegal XbaI site found at 1634
    Illegal PstI site found at 2359
    Illegal PstI site found at 2608
    Illegal PstI site found at 2630
    Illegal NgoMIV site found at 1322
    Illegal NgoMIV site found at 1479
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 3115


[edit]
Categories
Parameters
None