Part:BBa_K1179016

L7Ae (Transcriptional level repressor)

L7Ae is a transcriptional level repressor which targets RNA K-turns.

2019 SYSU-CHINA's characterization

SYSU-CHINA added some quantitative experimental characterization data on the L7Ae protein.
L7Ae is a protein targets RNA K-turn. We characterizated L7Ae and constructed an L7Ae mutation（L7AeO,BBa_3179001) to study the suppression efficiency of the L7Ae-Kturn System.

The result shows that the mutation couldn’t work normally but still functions in high concentration. Meanwhile, the 1000ng/ml L7Ae can depress the translation of mRNA with a efficiency of 84%.

Now the sequence analysis and computer modeling have shown the predicted structure of L7Ae. We can easily see that there are some α-helixes in L7Ae, and one of them is the bidding site.

Figure 3: L7Ae structure with K-turn from PDB

Cytotoxicity test

SYSU-CHINA tested the cytotoxicity of L7Ae, which was previously considered to be slightly cytotoxic, but the opposite result was found in our experiments.Neither L7Ae nor its mutants showed significant cytotoxicity.

Usage and Biology

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]