Generator

Part:BBa_K1179015

Designed by: Nelson Hall   Group: iGEM13_MIT   (2013-09-15)


A U6_gRNA(Cr9) plasmid which produces Cr9 targeting guide RNA's for Cas9

The U6 promoter is a transcriptional level promoter which leaves the transcribed sequence as RNA. This promoter then drives the production of guide RNA which targets Cas9 to Cr9 sites (Cr9 target sequence included below).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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