Coding

Part:BBa_K1179013

Designed by: Nelson Hall   Group: iGEM13_MIT   (2013-09-15)


eYFP (Yellow) fluorescent protein with a fused PEST site for rapid degradation

A yellow fluorescent protein with a PEST site, which marks the protein for rapid degradation. To be used when a reporter is needed to have a shorter half-life within the cell.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 205
    Illegal PstI site found at 812
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 205
    Illegal PstI site found at 812
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 205
    Illegal PstI site found at 812
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 205
    Illegal PstI site found at 812
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None