Coding

Part:BBa_K1179009

Designed by: Nelson Hall   Group: iGEM13_MIT   (2013-09-15)


(Cas9-VP16) A non-nuclease Cas9 with a C-terminal VP16 fusion for activation

This codes for a non-nuclease (DNA binding) Cas9 mutant with a C-terminal VP16 activating domain. It's capable of binding to DNA at upstream regulatory sites if provided the proper guide RNA to target said upstream regulatory sites.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3343
    Illegal PstI site found at 1732
    Illegal PstI site found at 1966
    Illegal PstI site found at 3178
    Illegal PstI site found at 3482
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3343
    Illegal PstI site found at 1732
    Illegal PstI site found at 1966
    Illegal PstI site found at 3178
    Illegal PstI site found at 3482
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3343
    Illegal BglII site found at 708
    Illegal BamHI site found at 1526
    Illegal XhoI site found at 3064
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3343
    Illegal PstI site found at 1732
    Illegal PstI site found at 1966
    Illegal PstI site found at 3178
    Illegal PstI site found at 3482
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3343
    Illegal PstI site found at 1732
    Illegal PstI site found at 1966
    Illegal PstI site found at 3178
    Illegal PstI site found at 3482
    Illegal NgoMIV site found at 2260
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None