Coding

Part:BBa_K1179007

Designed by: Nelson Hall   Group: iGEM13_MIT   (2013-09-15)


(Acyl-TyA-TCS-rtTA3-His) A membrane/exosome targeting rtTA3 fusion with a TEV clevage site

This fusion protein, when expressed, is targeted to the cell membrane and into exosomes and can thus be used to send rtTA3 from cell to cell. When expressed with TEV protease, the TEV sites is cleaved and releases the rtTA3 from the Acyl-TyA domain, relieving your fused protein from any steric effects of the Acyl-TyA domain.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 604
    Illegal PstI site found at 853
    Illegal PstI site found at 875
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 604
    Illegal PstI site found at 853
    Illegal PstI site found at 875
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 704
    Illegal BamHI site found at 1351
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 604
    Illegal PstI site found at 853
    Illegal PstI site found at 875
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 604
    Illegal PstI site found at 853
    Illegal PstI site found at 875
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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