Coding

Part:BBa_K1179005

Designed by: Nelson Hall   Group: iGEM13_MIT   (2013-09-15)


(Acyl-TyA-rtTA3-His) A membrane/exosome targeting rtTA3 fusion to send through exosomes with His tag

This fusion protein, when expressed, is targeted to the cell membrane and into exosomes and can thus be used to send rtTA3 from cell to cell and has an appended His tag for western blotting.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 604
    Illegal PstI site found at 853
    Illegal PstI site found at 875
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 604
    Illegal PstI site found at 853
    Illegal PstI site found at 875
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 704
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 604
    Illegal PstI site found at 853
    Illegal PstI site found at 875
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 604
    Illegal PstI site found at 853
    Illegal PstI site found at 875
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 537
    Illegal BsaI.rc site found at 889
    Illegal BsaI.rc site found at 1342


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