Reporter
Part:BBa_K1179003
Designed by: Nelson Hall Group: iGEM13_MIT (2013-09-15)
(Acyl-TyA-mkate-His) A membrane targeting mkate construct with an appended His tag
A membrane/exosome targeting Acyl-TyA protein fusion which can be assayed for by western blotting for the His tag.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 598
Illegal PstI site found at 847
Illegal PstI site found at 869 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 598
Illegal PstI site found at 847
Illegal PstI site found at 869 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 698
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 598
Illegal PstI site found at 847
Illegal PstI site found at 869 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 598
Illegal PstI site found at 847
Illegal PstI site found at 869 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1354
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Categories
Parameters
None |