Reporter

Part:BBa_K1179003

Designed by: Nelson Hall   Group: iGEM13_MIT   (2013-09-15)


(Acyl-TyA-mkate-His) A membrane targeting mkate construct with an appended His tag

A membrane/exosome targeting Acyl-TyA protein fusion which can be assayed for by western blotting for the His tag.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 598
    Illegal PstI site found at 847
    Illegal PstI site found at 869
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 598
    Illegal PstI site found at 847
    Illegal PstI site found at 869
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 698
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 598
    Illegal PstI site found at 847
    Illegal PstI site found at 869
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 598
    Illegal PstI site found at 847
    Illegal PstI site found at 869
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1354


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Categories
Parameters
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