Reporter

Part:BBa_K1179001

Designed by: Nelson Hall   Group: iGEM13_MIT   (2013-09-15)


(Acyl-TyA-eGFP) A membrane targeting eGFP construct

This reporter is used to visualize the membrane/exosome targeting ability of the Acyl-TyA sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1384
    Illegal SpeI site found at 1359
    Illegal PstI site found at 604
    Illegal PstI site found at 853
    Illegal PstI site found at 875
    Illegal PstI site found at 1389
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1384
    Illegal SpeI site found at 1359
    Illegal PstI site found at 604
    Illegal PstI site found at 853
    Illegal PstI site found at 875
    Illegal PstI site found at 1389
    Illegal NotI site found at 1410
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1384
    Illegal BglII site found at 704
    Illegal BamHI site found at 1353
    Illegal XhoI site found at 1417
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1384
    Illegal SpeI site found at 1359
    Illegal PstI site found at 604
    Illegal PstI site found at 853
    Illegal PstI site found at 875
    Illegal PstI site found at 1389
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1384
    Illegal SpeI site found at 1359
    Illegal PstI site found at 604
    Illegal PstI site found at 853
    Illegal PstI site found at 875
    Illegal PstI site found at 1389
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 537
    Illegal BsaI.rc site found at 889
    Illegal BsaI.rc site found at 1342


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