Intermediate

Part:BBa_K1179000

Designed by: Nelson Hall   Group: iGEM13_MIT   (2013-09-15)


(Acyl-TyA) A membrane targeting domain for exosomal transportation of proteins

Addition of a coding sequence after the Acyl-TyA coding sequence will produce a fusion protein which, when expressed, will be targeted to the cell membrane and into exosomes.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1410
    Illegal PstI site found at 643
    Illegal PstI site found at 892
    Illegal PstI site found at 914
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1410
    Illegal PstI site found at 643
    Illegal PstI site found at 892
    Illegal PstI site found at 914
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1410
    Illegal BglII site found at 743
    Illegal BamHI site found at 1
    Illegal BamHI site found at 1390
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1410
    Illegal PstI site found at 643
    Illegal PstI site found at 892
    Illegal PstI site found at 914
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1410
    Illegal PstI site found at 643
    Illegal PstI site found at 892
    Illegal PstI site found at 914
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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