Reporter

Part:BBa_K1165001

Designed by: iGEM Concordia 2013   Group: iGEM13_Concordia   (2013-09-17)

GFP Fluorescent Protein under the Control of the pOmpC Promoter

Description

A reporter gene (GFP) from Aequorea victoria that is induced via an osmotic stress input. Ompc promoter, from E. coli, is induced by interactions with phosphorylated OmpR protein, which is itself phosphorylated by the two-component histidine kinase, EnvZ, an osmotic sensor. This part's function within our biological cellular automaton was to characterize our EEHK fusion proteins (BBa_K1165006, BBa_K1165007, BBa_K1165008).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 778


[edit]
Categories
Parameters
None