Composite

Part:BBa_K1162014

Designed by: Ryan Putman   Group: iGEM13_Utah_State   (2013-09-17)


protmoter+rbs+N-terminal 10x His-Tag+GFP with no translational stop codon

N-terminal 10x His-Tag fused with GFP (no translational stop codon) to allow for N-terminal HT-GFP tagged protein fusion. RFC 23 allows for in frame fusion on the C-terminal end of GFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 269
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 788
    Illegal XhoI site found at 689
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None