Composite

Part:BBa_K1162013

Designed by: Kathleen Miller   Group: iGEM13_Utah_State   (2013-09-15)


promoter+rbs+N terminal His Tag and GFP

This part demonstrates that the N-terminal 10x His Tag functions as desired. It contains a promoter and rbs (BBa_K208010) with a GFP fusion (BBa_K208000). His Tag and GFP are RFC 23 compatible which allows for protein fusion with the His Tag.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 269
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 788
    Illegal XhoI site found at 689
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None