Part:BBa_K116201

ureD promoter from P mirabilis

An 328 bp promoter region, which can be assembled with urea binding proteins to activate the downstream gene expression.

The Manchester iGEM 2021 team has updated the sequence of this part in https://parts.igem.org/Part:BBa_K3710003 for our project UriGel.

Manchester iGEM 2021 UreD Description: pUreD is an inducible promoter that is activated by the UreR transcription factor in the presence of urea. pUreD is one of several promoters activated by the UreR transcriptional activator which share a consensus sequence of TA/GT/CA/TT/GC/TTA/TT/AATTG [3].

pUreD is a bidirectional promoter driving transcription of the UreR and UreD protein subunits of the urease operon of P. mirabilis. pUreD contains two ribosome binding sites and a UreR binding site about upstream of the forward transcriptional start site.

References:

[1] Poore, C. and Mobley, H. (2003) "Differential regulation of the Proteus mirabilis urease gene cluster by UreR and H-NS", Microbiology, 149(12), pp. 3383-3394. doi: 10.1099/mic.0.26624-0.

[2] Dattelbaum, J. et al. (2003) "UreR, the Transcriptional Activator of the Proteus mirabilis Urease Gene Cluster, Is Required for Urease Activity and Virulence in Experimental Urinary Tract Infections", Infection and Immunity, 71(2), pp. 1026-1030. doi: 10.1128/iai.71.2.1026-1030.2003.

[3]. Thomas V, Collins C. Identification of UreR binding sites in the Enterobacteriaceae plasmid-encoded and Proteus mirabilis urease gene operons. Molecular Microbiology. 1999;31(5):1417-1428.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]